Frequently Asked Questions Do I need to modify my HPLC system to use NPS columns? Easily Adaptable Start-up Install in Conventional Instrument Follow Simple Guidelines To Minimize Extra Column Volume Lower Organic Modifier & Filter Mobile Phase - 0.2µ Filter Enjoy The Immediate Saving In Time and Analysis Costs NPS® Column Guidelines Injection Volumes 0.5-10 µL typical, up to 100 µL possible Injection Mass <100 µg, solvent dependent Flow Rate Range 0.5 - 2.0 ml/min Normal Operating Pressure 1500 - 3500 psi (Based on 33 X 4.6 I.D. column) pH Range pH 2-9 Solvent Usage vs. 5µ Particle Column up to 95% less Speed vs. Conventional 5µ Column 3-10x Faster Analysis

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