Extending the concept of liquid fractionation using the ProteoSep technique Eprogen now produces whole proteome custom and standard microarrays for probing with antibodies. By collecting the liquid phase output from the second dimension analysis, discrete fractions containing small subsets of "intact" proteins contained in cell lysates (or subsellular fractions and biofluids) can be arrayed in a unique 3D format to serve as "bait" for probing with sera for autoantibody response (serological assays) or with known antibodies to mine for specific proteins.

Eprogen combines its ProteoSep Fractionation technique with Argonne National Lab's patented Gel-Drop technology to produce 3D BioChips where the proteins are encapsulated in a highly porous polymer matrix for enhanced interaction with antibodies eliminating the well documented problems of spotting microarrays on 2D surfaces. 

These BioChips work like "mini-secondary ELISA's" allowing researchers to use antibodies to probe for proteins (or patterns of proteins) of interest in complex samples thus serving as a unique complement to MS analysis and Western Blotting.  Standard cell lines or Custom cell lines can be easily arrayed and 1000's of BioChips produced for analysis using routine laboratory techniques for assaying and imaging slides.  A portion of the ProteoSep fractions are retained for later analysis of proteins contained in the "spots" of interest using MS Western Blots or other analytical techniques.

Please contact us to discuss your custom microarray printing needs.